Figure 3.

Comparison of the dual reporter system with the conventional CAT reporter system. The same promoter regions used in the dual reporter vector system were re-cloned into the CAT reporter system, pCAT®3-Enhancer vector (A). Vectors harboring different lengths of the promoter region were cotransfected with pSV-β-Galactosidase vector. The transcriptional activities of each region were determined via Chloramphenicol acetyl transferase activity assays (B, C) after compensation with beta-galactosidase. The pCAT3 control vector without the promoter sequence (pCAT®3-Basic) and the one harboring the SV40 promoter (pCAT®3-Control) were used as negative and positive controls, respectively.