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Cellular immune responses in proteins vaccine-immunized mice. Numbers of IFN-γ secreting cell spots from a pool of 4 mice splenocytes (4×105 cells/well) were determined by recall Ag VP1 or 3D protein stimulation. The splenocytes were pooled from 4 mice harvested 3 weeks after the last vaccination with VP1 and 3D protein. The ELISpot assay was performed after stimulating the cells with 10 µg/ml each of the recombinant proteins for 48 hrs.
