Table 2.
Gene regions | PCR primers | Additional primers used for sequencing |
---|---|---|
nucLSU | LR0Ra, LR7b | LR3, LR3R, LR5, LR5R, LR6, LR6Rb |
nucSSU | nssu131c, NS24d | nssu1088, nssu1088R, nssu897R, nssu634c, SR11Re, NS23, NS22d, SR7R, SR7, SR10Rf |
mtSSU | mtSSU1, mtSSU3Rg | mtSSU2, mtSSU2Rg |
RPB1 region A—D | RPB1-AFh, RPB1-6R1asci | — |
RPB2 region 5-7 | RPB2-5F, RPB2-7cRj | — |
RPB2 region 7-11 | RPB2-7cF, RPB2-11aRj | — |
Rehner & Samuels (1994), bVilgalys & Hester (1990), cKauff & Lutzoni (2002), dGargas & Taylor (1992), eSpatafora et al. (1995), fVilgalys (unpubl.; www.biology.duke.edu/fungi/mycolab/primers.htm), gZoller et al. (1999), hHall (unpubl.; http://faculty.washington.edu/benhall/), iHofstetter et al. (2007), jLiu et al. (1999).