Figure 1.
Generation of Pig-a−/− mice. Mice carrying the Pig-aflox construct were mated with mice carrying the Cre recombinase transgene driven by the Fes promoter to produce WT (Pig-a+/+), heterozygous (Pig-a+/−), homozygous Pig-a−/− and hemizygous (Pig-a−/0) mice. (A) PCR analysis identified a 230 bp band for WT mice and a 350 bp band for the knock-out (Pig-a−/− Pig-a, −/0) mice while heterozygous (Pig-a+/−) mice had both PCR fragments. All mice were positive for the Cre recombinase. (B) A representative Facs plot shows the comparison between a Pig-a+/− and a female Pig-a−/− animal highlighting that the leukocytes of Pig-a−/− mice contain a fraction of cells that are negative for the CD48 antigen.