Figure 1.

Temperature-dependent macronutrient intake during the fifth stadium. (a) Self-selected (choice) cumulative intake trajectories (bivariate means with standard errors; corrected for insect initial mass) of insects through protein–carbohydrate (P–C) space at 26, 32 and 38°C. For each time course, points moving away from the origin (labelled in the case of 38°C) represent cumulative intake on days 2, 4, 6 and day-of-molt, respectively. Large white circles, stadium intake target. (b) Comparison of stadium intake between insects restricted to diets (no-choice groups representing P : C, 32 : 10 and 10 : 32; diagonal lines from origin) with intake from insects allowed continuous access to both diets (choice groups self-selecting positions in the intervening P–C space) at 26, 32 and 38°C. (c) Diverse daily mean rates and standard errors of P and C intake by choice and no-choice insects at 26, 32 and 38°C. n = 30 for each temperature; insects failing to moult were excluded from further analysis. Small white circles, 26°C; grey circles, 32°C; black circles, 38°C.