Figure 6.

Treatment with US at 42 KHz and 0.15 W/cm² does not change phenotypic maturation of DC. DC were generated from peripheral blood monocytes cultured with GM-CSF and IL-4 for 6 days. At day 5, immature DC were treated or not with 3 cycles of US, and LPS (50 μg/mL) was added to promote maturation. After 24 hours incubation, DC were stained with FITC-conjugated Abs against the indicated surface molecules. Thin and bold black lines indicate US-treated or untreated mature DC, respectively. Dotted lines represent staining with matched isotype immunoglobulins. The x-axis and the y-axis indicate the relative cell number and fluorescence intensity, respectively.