. 2010 Jan 20;2009:567120. doi: 10.1155/2009/567120

Table 2.

Descriptions of the 33 primers used in this study.

Gene	Primer	Forward/reverse	Sequence (5′ to 3′)	Annealing temp (°C)	Purpose
cGHRL	PM1	PM1F	tgctgaaggaccgaaaacaaa	56	SNP identification
	PM1	PM1R	gccttgacagatgccttagtg	56	SNP identification
	PM2	PM2F	aagaaagctggtaactgcactag	55	SNP identification
	PM2	PM2R	ggtgggctggtggagtta	55	SNP identification
	PM3	PM3F	tgcgttctgctactctttttcat	63	SNP identification
	PM3	PM3R	gggccaaggaggagtgtct	63	SNP identification
	PM4	PM4F	cggcagacactcctcctt	57	SNP identification
	PM4	PM4R	gccatccttccaactgtgtatat	57	SNP identification
	PM5	PM5F	tatgcgttctgctactcttt	58	Genotyping by sequencing
	PM5	PM5R	tggaagcgatcactatacc	58	Genotyping by sequencing
	PM6	PM6F	catacagcaacaaaaggatac	63	Real time PCR
	PM6	PM6R	tgtggttgtccttcagct	63	Real time PCR

cGHSR	PM7	PM7F	gtgggtcagggcatcaaactc	58	SNP identification and PCR-RFLP
	PM7	PM7R	tcgagggctgctgaattttatg	58	SNP identification and PCR-RFLP
	PM8	PM8F	ggctcttcctttttggtttgtct	60	SNP identification and PCR-RFLP
	PM8	PM8R	tcgccctctctctgattcacct	60	SNP identification and PCR-RFLP
	PM9	PM9F	tgatgccactggtctgagaat	58	Genotyping by PCR-RFLP
	PM9	PM9R	tatccagctgcccatgtaaat	58	Genotyping by PCR-RFLP
	PM10	PM10F	tgggcgtcgagcatgagaat	63	Real time PCR
	PM10	PM10R	ccacgactagcatcttcacag	63	Real time PCR

Chicken β-actin	PM11	PM11F	ccccaaagccaacagagaga	63	Real time PCR
Chicken β-actin	PM11	PM11R	ggtggtgaagctgtagcctctc	63	Real time PCR

dGHRL	PM12	PM12F	Genomic Walking Kit	According to the kit	Genomic Walking
		PM12R1	ttccatcagttctagacgagt
		PM12R2	tgtttgtccatactcttgatact
		PM12R3	gcccctgctttatctgtatc
	PM13	PM13F	ccgcctggtgaagaaaac	56	RT-PCR
	PM13	PM13R	aagcctacacatccacctgcaat	56	RT-PCR
	PM14	PM14F	gaggcaagctgaaggacaaccaca	54	3′ RACE
	PM14	PM14R	3′ RACE Kit	54	3′ RACE
	PM15	PM15F	gctggttttcccgtgtaattc	56	RT-PCR
	PM15	PM15R	tgtttgtccatactcttgatact	56	RT-PCR
	PM16	PM16F	gctggttttcccgtgtaattc	54	Intron 1 amplification
	PM16	PM16R	tgtttgtccatactcttgatact	54	Intron 1 amplification
	PM17	PM17F	tggtttggctggctctagt	56	Intron 2 amplification
	PM17	PM7R	PM12R3	56	Intron 2 amplification
	PM18	PM18F	aaagcaggggcagaagat	57	Intron 3 amplification
	PM18	PM18R	PM12R2	57	Intron 3 amplification
	PM19	PM19F	agggtcctggtccaaaaat	54	Intron 4 amplification
	PM19	PM19R	PM12R1	54	Intron 4 amplification
	PM20	PM20F	cgcatggtagccttcacacac	56	SNP identification and PCR-RFLP
	PM20	PM20R	agccgatgggttagcagagag	56	SNP identification and PCR-RFLP
	PM21	PM21F	tcggctccatcagttgcagttat	56	SNP identification
	PM21	PM21R	cggcgatgtatttttgctgttg	56	SNP identification
	PM22	PM22F	tccccacgacagagtagtttgag	56	SNP identification and PCR-RFLP
	PM22	PM22R	gccttcccctgcttcctaa	56	SNP identification and PCR-RFLP
	PM23	PM23F	atagcagcattttagaagtga	54	SNP identification
	PM23	PM23R	ttgcctcagcttttcact	54	SNP identification
	PM24	PM24F	cgtgtaattcctctctgctaa	63	Real time PCR
	PM24	PM24R	cgatgtatttttgctgttgtt	63	Real time PCR

dGHSR	PM25	PM25F	cccctgagcaccaacgagt	56	Intron 1 amplification
	PM25	PM25R	ggcaaccagcagagtatga	56	Intron 1 amplification
	PM26	PM26F	Genomic Walking Kit	According to the kit	Genomic Walking
		PM26R1	cggaccgatgttctttctcttt
		PM26R2	gacgggcaggaaaaagaagac
		PM26R3	ggcccagaggatgaggatga
	PM27	PM27F	tggcgttctccgacctgctcat	57	SNP identification
	PM27	PM27R	acacagaccctcagaaacac	57	SNP identification
	PM28	PM28F	cgggtggcttaaataacaacag	56	SNP identification
	PM28	PM28R	tgccctttttcctcagtttctc	56	SNP identification
	PM29	PM29F	cggaccataattaaacacctgaa	56	SNP identification
	PM29	PM29R	caggtagaagaggacaaaggaca	56	SNP identification
	PM30	PM30F	tggcgttctccgacctgctcat	56	Genotyping by PCR-RFLP
	PM30	PM30R	acacagaccctcagaaacac	56	Genotyping by PCR-RFLP
	PM31	PM31F	gcaggttgtttagatatggct	56	Genotyping by PCR-RFLP
	PM31	PM31R	cgtgaaaaggcaaccagcagag	56	Genotyping by PCR-RFLP
	PM32	PM32F	cccctgagcaccaacgagt	63	Real time PCR
	PM32	PM32R	ccaccacaactaacattttcaca	63	Real time PCR

Duck β-actin	PM33	PM33F	acgccaacacggtgctg	63	Real time PCR
Duck β-actin	PM33	PM33R	gggtccggattcatcatactc	63	Real time PCR