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Effects of neonatal lipopolysaccharide (LPS; 50 μg/kg i.p.) at postnatal day (pnd) 3 + 5, 7 + 9 or 14 + 16 on the timing of vaginal opening (a) or first vaginal oestrus (b). Data are presented as the delay, in days, in vaginal opening or first oestrus due to neonatal-LPS treatment relative to neonatal saline treated controls. Only the neonatal-LPS treated rats on pnd 3 + 5 showed a significant delay in pubertal onset (P < 0.05). (mean ± SEM; n = 12–26 per group).
