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. 2009 Aug;21(8):683–689. doi: 10.1111/j.1365-2826.2009.01885.x

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Journal compilation © 2009 Blackwell Publishing

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Fig. 2 — Effects of neonatal lipopolysaccharide (LPS, 50 μg/kg i.p.) or saline control at postnatal day (pnd) 3 + 5 on kisspeptin (Kiss1) mRNA expression in the medial preoptic area (mPOA) (a) and arcuate nucleus (ARC) (c), and on kisspeptin receptor (Kiss1r) mRNA expression in the mPOA (b) and ARC (d) in female rats at pnd 14, pnd 32, the day of vaginal opening (dVO) and at 11 weeks of age (Adult). Kiss1 and Kiss1r mRNA levels were measured in brain micropunch samples from the mPOA or ARC using real-time reverse transcriptase-polymerase chain reaction. Quantification for Kiss1, Kiss1r and 28S rRNA was carried out on all samples; the values are expressed as a ratio of Kiss1 mRNA and 28S rRNA, or Kiss1r mRNA and 28S rRNA (mean ± SEM). *P < 0.05 versus the respective treatment group at different time points; ^#P < 0.05 versus saline control at same time point; n = 5–9 per group.