Table 1.
Oligonucleotides used in RNase H assays and Tm data
| Name | Sequence (5′–3′)a |
Tm (°C)b |
|
|---|---|---|---|
| DNA-1 | RNA-2 | ||
| RNA-1 | GAU CUG AGC CUG GGA GCU | 65.1 | 78.5 |
| PhpC-1 | GAU CUG AGC CUG GGA G*CU | 64.7 | 78.8 |
| PhpC-2 | GAU CUG AGC *CUG GGA GCU | 67.5 | 80.8 |
aPhpC indicated by *C.
bTm values represent the average of at least 3 independent experiments within 1°C.