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. 2009 Nov 20;38(3):1048–1056. doi: 10.1093/nar/gkp1022

Table 1.

Oligonucleotides used in RNase H assays and Tm data

Name Sequence (5′–3′)a Tm (°C)b
DNA-1 RNA-2
RNA-1 GAU CUG AGC CUG GGA GCU 65.1 78.5
PhpC-1 GAU CUG AGC CUG GGA G*CU 64.7 78.8
PhpC-2 GAU CUG AGC *CUG GGA GCU 67.5 80.8

aPhpC indicated by *C.

bTm values represent the average of at least 3 independent experiments within 1°C.