Table 2.

Fluorescent properties of PhpC and oligonucleotides containing single PhpC inserts

Entry	Name	Fluorescence intensitya	Quantum yieldb Φ	FPc
1	PhpC	51	0.31	<0.02
2	5′-PO4-PhpC	47	0.29	<0.02
3	G-PhpC-U	36	0.11	0.03
4	C-PhpC-U	104	0.24	0.03
5	U-PhpC-U	134	0.41	0.03
6	A-PhpC-U	106	n.d.	0.03
7	PhpC-1	35	0.11	0.21
8	PhpC-1:DNA-1	7.8	0.035	0.28
9	PhpC-1:RNA-2	9.5	n.d.	0.30
10	PhpC-2	38	0.13	0.22
11	PhpC-2:DNA-1	80	0.20	0.27
12	PhpC-2:RNA-2	69	n.d.	0.28

^aOne micro molar samples measured in 10 mM phosphate buffer (pH 7.0) and 50 mM NaCl at 25°C, λ_ex = 360 nm; λ_emm = 465 nm

^bSee Supporting Information for Quantum Yield determination.

^cFP measured in identical conditions as footnote a.

n.d. signifies not determined.