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. 2009 Nov 12;38(3):e15. doi: 10.1093/nar/gkp1025

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© The Author(s) 2009. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — Toeprint analysis of a ribosome–mRNA complex formation. (A) Stable ribosomal (RS) complexes stop reverse transcriptase (RT) at a certain mRNA position generating short cDNA products of specific lengths. Comparison of a (B) classical toeprint experiment—electrophoretic analysis of radioactively labeled products of the primer extension inhibition reaction and (C) alternative approach utilizing fluorescently labeled primers and capillary electrophoresis for detection of the cDNA products. Peaks of fluorescence correspond to the bands on radioautograph.