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. 2009 Nov 12;38(3):e15. doi: 10.1093/nar/gkp1025

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© The Author(s) 2009. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Detection of the initiation ribosome–mRNA 48S complex on natural β-globin mRNA. Lower curves show the case where 40S ribosomal subunits, eIFs: 1, 1A, 2, 3, 4A, 4B, 4F and Met- were present in the reaction mixture. Upper curves are the result of a control reaction where eIF2 was excluded. (A) The original fluorescence readout of the capillary electrophoresis. (B) Same as (A), but the cDNA peaks of fluorescence (blue line) were aligned with the mRNA sequence using Promega CXR size standards (red line). (C) Scaled region of (B) between the 5′-end and toeprint signals, normalized by the integral fluorescence intensity. The aligned β-globin mRNA sequence is shown in the bottom. Three main electrophoregram zones are indicated (see text).

Inline graphic — Detection of the initiation ribosome–mRNA 48S complex on natural β-globin mRNA. Lower curves show the case where 40S ribosomal subunits, eIFs: 1, 1A, 2, 3, 4A, 4B, 4F and Met- were present in the reaction mixture. Upper curves are the result of a control reaction where eIF2 was excluded. (A) The original fluorescence readout of the capillary electrophoresis. (B) Same as (A), but the cDNA peaks of fluorescence (blue line) were aligned with the mRNA sequence using Promega CXR size standards (red line). (C) Scaled region of (B) between the 5′-end and toeprint signals, normalized by the integral fluorescence intensity. The aligned β-globin mRNA sequence is shown in the bottom. Three main electrophoregram zones are indicated (see text).