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. 2009 Nov 12;38(3):e15. doi: 10.1093/nar/gkp1025

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© The Author(s) 2009. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 9. — Detection of the 48S complex assembly sites in the mixture of natural β-globin mRNA (FAM fluorescence, blue line) and synthetic A₂₅-Luc mRNA (JOE fluorescence, red line). Both fluorescence readouts from FAM and JOE are normalized by the integral fluorescence intensity of the corresponding wavelength. The aligned β-globin and A₂₅-Luc mRNA sequences are shown in the bottom. Complex-specific electrophoregram zones are indicated, and relative yield of the 48S complex assembled on the corresponding mRNA is shown.