Table 6.
The results of experimental validation of miRNA–target interactions in three samples
| miRNAs | Target gene ID | Target gene function | Cleavage sitea |
||
|---|---|---|---|---|---|
| HeLa-ck | HeLa-stress (cold+drought) | SC124-cold recovery | |||
| 160ab | 27538.m000327 | Auxin response factor | 10th 12/13) | 10th (11/11) | 10th (13/13) |
| 393b | 29647.m002022 | Transport inhibitor response1 protein | 11th(10/10) | 11th(9/9) | |
| 164 | 30138.m004055 | NAC domain-containing protein 21/22 | 10th(8/8) | 10th(7/7) | 10th (7/7) |
| 156e | 30147.m014478 | Squamosa promoter -binding protein | 9th(1/3), 10th(1/3) | 9th(4/6) | 10th (2/5) |
| 156e | 30174.m009082 | LIGULELESS1 protein | −1st(1/7), 6th(5/7), 9th(1/7) | 9th(8/13), 10th(5/13) | 9th (3/11), 10th (8/11) |
| 156e | 29269.m000250 | LIGULELESS1 protein, putative | 9th(5/8), 10th(1/8) | 9th(2/7) | 10th (2/7) |
| 160ab | 27494.m000045 | Auxin response factor | |||
| 393b | 30131.m006863 | Transport inhibitor response1 protein | |||
| 171e | 30174.m008828 | Hypothetical protein | |||
| 399a | 30026.m001455 | Conserved hypothetical protein | |||
aThe detailed miRNA–guided cleavage of target mRNAs, the number of cleavage position is counted based on the mature miRNA sequence from the 5′- to 3′-end, the numerator and denominator of fraction to indicate the number of individuals and the whole clones which were cloned to vector and achieve the sequence.
The three-sample conditions listed as following:
HeLa-cold/drought ck: a mixture of equal quantity of HeLa-cold ck and HeLa-drought ck.
SC124-cold recovery: SC124 returning back to 25°C after 5 days cultivation under 14°C.
HeLa-stress: a mixture of equal quantity of HeLa-cold and HeLa-drought.