Figure 3.

Binding titrations of 14-3-3 τ and σ and fluorescein-labeled peptides. (A) Binding titration of 14-3-3 τ and σ to mode-2 consensus peptide. (B and D) Binding of σ isoform to mono- and di-phosphorylated p53 CTD peptides. (C and E) Binding of τ isoform to mono- and di-phosphorylated p53 CTD peptides. Peptides sequences are listed in Table 2. Experiments were carried out at 20°C in 20 mM HEPES, 150 mM NaCl, 5 mM DTT, pH 7.2. Titrations of γ and ε to p53CTD phosphopeptides were reported earlier (13).