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Schematic drawing of the structure of the 5′-UTR of human Nrf2 mRNA and plasmid constructs. Wild-type (WT) or various deleted mutants of 5′-UTR of Nrf2 mRNA were subcloned into the intercistronic region of a bicistronic vector containing a Rluc and a Fluc. 5′-UTR: 5′-untranslated region; (A)n: poly(A) tail; IE: inhibitory element; ORF: open reading frame; RBS: ribosomal binding site; TSS: transcription start site.
