Table 1.

ChIP assay on putative HREs in the promoter of five NER factors

Genes	Sequence 5′→3′	Location	Relative fold enrichmenta
			Ker/NT-nIr	Ker/NT-10 h
XPB	ggCGTGag	−3960/–3953	1.1 ± 0.3	2.9 ± 0.4
	caCGTGgt	−3104/–3097	1.5 ± 0.4	0.9 ± 0.5
	ggCGTGat	−1647/–1640	0.8 ± 0.6	1.1 ± 0.4
	ggCGTGag	−1431/–1424	1.2 ± 0.5	1.1 ± 0.3
	ggCGTGag	−379/–372	3.8 ± 0.6	4.5 ± 0.7
XPF	ggCGTGag	−2201/–2194	1.1 ± 0.3	1.3 ± 0.2
	gaCGTGaa	−380/–373	1.9 ± 0.5	1.5 ± 0.4
XPG	ggCGTGag	−3314/–3037	1.1 ± 0.6	2.2 ± 0.6
	ggCGTGgt	−1898/–1891	0.9 ± 0.5	1.2 ± 0.4
	taCGTGct	−832/–825	4.0 ± 0.3	4.5 ± 0.6
CSA	cgCGTGcc	−2590/–2583	1.1 ± 0.4	1.0 ± 0.5
	ggCGTGgc	−642/–635	0.8 ± 0.3	1.1 ± 0.4
	caCGTGct	−276/–269	3.0 ± 0.5	5.0 ± 0.7
CSB	ggCGTGgt	−1267/1260	0.9 ± 0.2	1.6 ± 0.3
	taCGTGga	−270/–263	1.2 ± 0.3	1.1 ± 0.4
	caCGTGgt	−104/–97	5.0 ± 0.6	4.0 ± 0.8

^aTen hours after UVB-exposure, irradiated and non-irradiated keratinocytes were subjected to ChIP assay using an anti-HIF-1α antibody. The relative levels of corresponding precipitated HRE fragments following ChIP were quantified by qRT-PCR using primers that span the indicated HREs. The PCR amplification in shHIF-1α-transduced cells detected a minimal level of HREs which was normalized to one for each experiment. The results are expressed as the mean ± SD of three independent experiments.