Table 1.
Sequences of siRNAs, primers and probes used in this study
| siRNAs | ||
| Name | Passenger strand | Guide strand |
| ApoB-1 | GUCAUCACACUGAAUACCAAU | AUUGGUAUUCAGUGUGAUGAmC*mA*C |
| ApoB1 mm control | GUGAUCAGACUCAAUACGAAU | AUUCGUAUUGAGUCUGAUCAmC*mA*C |
| RRM1-2 | CCCAGUUACUGAAUAAGCAGAUCUU | AAGAUCUGCUUAUUCAGUAACUGGGCU |
| RRM1-3 | GCAAACUCACUAGUAUGCACUUCUA | UAGAAGUGCAUACUAGUGAGUUUGCCU |
| RRM1-15 | GAUUGUAAAUCCUCACUUAdTdT | UAAGUGAGGAUUUACAAUCdTdT |
| YBX1-9 | CCAGUUCAAGGCAGUAAAUdTdT | AUUUACUGCCUUGAACUGGdTdT |
| qPCR Primers | ||
| Target | Forward | Reverse |
| ApoB | GGCACTGTGGGTCTGGAT | TTCTTCTCTGGAGGGGACTG |
| RRM1 | GGCAAACTCACTAGTATGCACTTC | AAATAATACATCCCAGTCTTCAAACC |
| YBX1 | GGAGTTTGATGTTGTTGAAGGA | AACTGGAACACCACCAGGAC |
| Polr2a | TTACTCCCCTGCATGGTCTC | TGGGAGACATAGCACCACCT |
| LMNA | TGAGGCCAAGAAGCAACTTCA | CTCATGACGGCGCTTGGT |
| Gene-specific primers for first-strand cDNA synthesis | ||
| ApoB | AGAACCCGTGATTCAACCTG | |
| RRM1 | AAGCAGTGCTAAAGGGGTGA | |
| YBX1 | CCGGATGATGGTAGAGATGG | |
| First-round RACE gene-specific primers | ||
| ApoB | GCTCCCATGTGGTGTAGATGCGTTGGA | |
| RRM1 | TGCTGCATTTGATGGTTCCCAGGTTCTG | |
| YBX1 | TCTGGGCGTCTGCGTCGGTAATTGA | |
| MBRACE primers | ||
| Target site | Forward | Reverse |
| ApoB-1 | ACTGGAGCACGAGGACACTG | GGAAGAAAGGAAATGGGCAACG |
| RRM1-2 | CGACTGGAGCACGAGGAC | AGCCCTCATAGGTTTCGTATGG |
| RRM1-3 | CGACTGGAGCACGAGGAC | GATTAGCCGCTGGTCTTGTC |
| RRM1-15 | ACTGGAGCACGAGGACAC | AGGAATTTCTGGTATGCTCTG |
| YBX1-9 | CGACTGGAGCACGAGGACAC | TGCTGGTAATTGCGTGGAGGAC |
| Molecular beacon probes | ||
| ApoB-1 | F-CGCGATCCCAGCATTGGTATTCTTTCTACTCCTTCAGATCGCG-B | |
| RRM1-2 | F-CGCGATCAAAGATCTGCTTTTCTACTCCTTCAGTCCGATCGCG-B | |
| RRM1-3 | F-CGCGATCCCGTAGAAGTGCATTTCTACTCCTTCAGATCGCG-B | |
| RRM1-15 | F-CGCGATCAGATCTTTCAATAAGTGAGGATTTCTACTCCGATCGCG-B | |
| YBX1-9 | F-CGCGATCTGCATATTTACTGCCTTTCTACTCCTTCAGATCGCG-B | |
All sequences are given in 5′-3′ orientation. The siRNA sequences are RNA except dTdT deoxynucleotides ends. The Stealth duplexes (RRM1-2 and RRM1-3) are dsRNA with proprietary modifications of the passenger strand; m, 2′-O-Methyl ribose-modified nucleotides; *phosphorothioate linkage; F, FAM dye label; B, Black Hole Quencher.