Skip to main content
. 2009 Dec 1;38(3):958–969. doi: 10.1093/nar/gkp927

Figure 2.

Figure 2.

Schematic model of how the plasmid containing the attDOT site orients itself relative to the attB to maintain alignment of the GC dinucleotide. (A) Orientation of wild-type attDOT and wild-type attB in the standard integration reaction. The vertical arrow indicates the site of initial cleavage. (B) Possible orientation of wild-type attDOT and the attB site containing an inverted overlap region. (C) Products from the attDOT and attB sites oriented as shown in B if the initial cleavage occurs at the location of the vertical arrow. There is complete heterology within the entire seven base overlap region in both products. (D) Rotation of the inverted overlap attB site by 180° orients the GC dinucleotide in the same position as in the wild-type reaction. The vertical arrow denotes the site of initial cleavage producing the recombinants that both contain the complementary GC dinucleotide on the left side.