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. 2009 Dec 1;38(3):958–969. doi: 10.1093/nar/gkp927

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© The Author(s) 2009. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 8. — (A) Sequence comparison of wild-type attDOT and an attDOT site with a terminal G mutation in the overlap region, and wild-type attB with an attB site containing substitutions at both ends of the overlap region. (B) Sequences of products formed from the recombination of wild-type or TermG attDOT with either wild-type or AT attB. The second pair of sequences in each combination is the products from an inverted attB site. Complementary base pairs are in bold. (C) Integration assay results from combinations of wild-type or TermG attDOT sites with either wild-type or AT attB sites. The average recombination was taken over a minimum of four experiments.