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SSTR5 protein expression in PBMCs of CD patients and healthy donors activated or not by LPS. To mimic inflammation, PBMCs were incubated in presence or absence of LPS (1 μg/mL) for 2 hours 30 minutes at 37°C. The cells were fixed and permeabilized and SSTR5 revealed by an Alexa 488 coupled antibody. The histogram (b) shows that after LPS activation of healthy PBMCs, all cells were presenting SSTR5 proteins, in the same way that PBMCs from CD patients activated (d) or not (c) by LPS.
