Fig. 2.

Steady state activation and inactivation curves of I_Ca(L) obtained before and after LPC application. (A) Steady state activation curves of I_Ca(L) in control and LPC. Chord conductance (G) was measured at different membrane potentials and normalized to the maximal chord conductance (G_max). Each data points were the means of 8 experiments and fitted using a following form of Boltzman equation; Y={1+exp[(V_1/2 - V)/k]}^-1, where V_1/2 represents half maximal activation potential and k is slope factor. Measured V_1/2 was -5.8±0.2 mV and -3.1±0.1 mV in control and LPC, respectively. (B) Steady state inactivation curves of I_Ca(L) in control and LPC. Current amplitudes (I) of test potential to 0 mV from different pretest potentials were normalized to the maximal current (I_max). Each data points were the means of 6 experiments. Curves were obtained from following form of Boltzman equation; Y={1+exp[(V - V_1/2)/k]}^-1, where V_1/2 represents half maximal inactivation potential and k is slope factor. V_1/2 was -31.4±1.2 mV and -30.2±1.6 mV in control and LPC, respectively.