Figure 4.
Mitochondrial UCP1 in BAT. Protein from IBAT of wild-type (wt), heterozygous, and homozygous TRβ PV mutant mice made hypothyroid by treatment with a low-iodine diet and propylthiouracil for 3 wk and then treated with daily ip doses of T3 (7 ng/g BW) or a saline vehicle control for 10 d. A, Western blot of mitochondrial protein using anti-UCP1 antibody; B, blots were analyzed by densitometry, and the results are shown. *, P < 0.01 vs. wild type; +, P < 0.01 vs. heterozygous. Values are the mean ± sd of three to four mice.