Fig. 3.

Rat FADS3 antibody characterization. To characterize the immunospecificity, antibodies were tested on rat recombinant FADS1, FADS2, and FADS3. Cos-7 cells were transfected with pCMV, pCMV/Fads1, pCMV/Fads2, or pCMV/Fads3 plasmids. Western blots were assessed on cell extracts containing recombinant FADS proteins. One polyclonal antibody detected both FADS2 and FADS3 and was referred to as anti-FADS2/3. Two purified polyclonal antibodies detected specifically FADS3 and were referred to as anti-NtermFADS3 and anti-Cterm-FADS3. Experiments were reproduced three times; results exhibit only one case in point.