TABLE 1.

Esterified fatty acid concentrations in brain phospholipid classes from n-3 PUFA adequate and deprived rats, measured following 15 weeks of feeding

	Concentration, µmol/g Brain
Esterified Fatty Acid	ChoGpl		EtnGpl		PtdIns		PtdSer		Total PL
	Adq.	Dep.	Adq.	Dep.	Adq.	Dep.	Adq.	Dep.	Adq.	Dep.
14:0	0.1 ± 0.09	0.12 ± 0.02	0.3 ± 0.09	0.3 ± 0.17	0.02 ± 0.003	0.03 ± 0.004	0.03 ± 0.002	0.01 ± 0.005**	0.5 ± 0.1	0.9 ± 0.2
16:0	17.7 ± 0.9	20.3 ± 1.8	3.7 ± 0.2	4.3 ± 0.2*	0.6 ± 0.03	0.7 ± 0.06	0.35 ± 0.04	0.35 ± 0.06	21.7 ± 1.3	23.1 ± 0.2
18:0	5.8 ± 0.3	6.8 ± 0.6	8.1 ± 0.4	9.2 ± 0.4	1.8 ± 0.1	1.9 ± 0.1	6.1 ± 0.2	6.4 ± 0.4	22.1 ± 1.1	22.8 ± 0.3
20:0	0.1 ± 0.006	0.1 ± 0.01	0.2 ± 0.05	0.1 ± 0.009	0.02 ± 0.003	0.02 ± 0.002	0.07 ± 0.006	0.06 ± 0.004	0.7 ± 0.03	0.9 ± 0.1
22:0	0.1 ± 0.01	0.2 ± 0.02	ND	0.05 ± 0.005	ND	ND	0.09 ± 0.003	0.09 ± 0.009	0.78 ± 0.04	0.8 ± 0.04
16:1n-9	0.3 ± 0.08	0.60 ± 0.2	0.6 ± 0.2	0.8 ± 0.3	0.02 ± 0.008	0.07 ± 0.02	0.02 ± 0.004	0.02 ± 0.003	1.6 ± 0.2	2.1 ± 0.3
18:1n-(7+9)	11.3 ± 1	13.0 ± 1.6	8.0 ± 0.9	9.8 ± 0.9	0.7 ± 0.05	0.7 ± 0.03	3.5 ± 0.3	3.5 ± 0.2	21.5 ± 1.0	21 ± 0.3
20:1n-9	0.5 ± 0.01	0.6 ± 0.05	1.4 ± 0.09	1.5 ± 0.06	0.06 ± 0.009	0.06 ± 0.002	0.4 ± 0.05	0.4 ± 0.03	1.1 ± 0.3	1.4 ± 0.3
22:1n-9	0.08 ± 0.01	0.09 ± 0.01	0.08 ± 0.005	0.09 ± 0.005	0.04 ± 0.01	0.09 ± 0.03	0.07 ± 0.005	0.08 ± 0.006	0.3 ± 0.1	0.4 ± 0.05
24:1n-9	0.1 ± 0.009	0.2 ± 0.02*	0.03 ± 0.002	0.06 ± 0.006**	0.01 ± 0.002	0.01 ± 0.002	0.03 ± 0.001	0.06 ± 0.007**	2.0 ± 0.16	2.3 ± 0.07
18:2n-6	0.3 ± 0.02	0.3 ± 0.03	0.13 ± 0.01	0.27 ± 0.04**	0.02 ± 0.002	0.02 ± 0.003	0.04 ± 0.004	0.04 ± 0.004	0.7 ± 0.16	0.6 ± 0.06
18:3n-3	0.027 ± 0.004	0.05 ± 0.008*	0.02 ± 0.0007	0.04 ± 0.007*	ND	ND	0.02 ± 0.006	0.02 ± 0.004	0.04 ± 0.02	0.02 ± 0.004
18:3n-6	ND	ND	0.008 ± 0.001	0.01 ± 0.006	ND	ND	0.004 ± 0.0001	ND	0.04 ± 0.009	ND
20:3n-3	0.05 ± 0.004	0.06 ± 0.007	0.09 ± 0.01	0.07 ± 0.004	ND	0.006 ± 0.001	0.02 ± 0.002	0.02 ± 0.004	0.07 ± 0.03	0.1 ± 0.02
20:4n-6	2.0 ± 0.1	2.4 ± 0.2	4.4 ± 0.03	5.2 ± 0.03 *	1.5 ± 0.1	1.7 ± 0.04	0.5 ± 0.07	0.6 ± 0.1	7.8 ± 0.3	8.9 ± 0.1*
22:4n-6	0.3 ± 0.01	0.4 ± 0.04**	1.9 ± 0.1	2.5 ± 0.09**	0.09 ± 0.015	0.1 ± 0.01	0.5 ± 0.04	0.6 ± 0.04	1.5 ± 0.3	1.3 ± 0.05
22:5n-6	0.04 ± 0.003	0.6 ± 0.05***	0.1 ± 0.01	2.3 ± 0.1***	0.01 ± 0.001	0.06 ± 0.006***	0.08 ± 0.04	1.2 ± 0.09***	0.2 ± 0.006	4.0 ± 0.2***
22:5n-3	0.03 ± 0.0001	0.01 ± 0.002***	0.09 ± 0.005	0.04 ± 0.001***	ND	ND	0.03 ± 0.001	0.02 ± 0.002***	0.14 ± 0.02	0.12 ± 0.02
22:6n-3	1.5 ± 0.1	1.0 ± 0.09**	6.2 ± 0.5	4.5 ± 0.09**	0.2 ± 0.04	0.1 ± 0.007*	2.6 ± 0.1	1.7 ± 0.07***	9.9 ± 0.5	7.3 ± 0.2***
Totalsa	40.3 ± 2.7	46.8 ± 4.5	35.3 ± 2.6	41.1 ± 1.9	5.1 ± 0.4	5.6 ± 0.3	14.5 ± 0.9	15.2 ± 0.9	92.7 ± 5.7	98.0 ± 2.0

Data are means ± SE (n = 5 independent samples per group). Total PL, total phospholipids; ND, not detected. *P < 0.05, **P < 0.01, ***P < 0.001; significant difference between n-3 PUFA deprived (Dep.) and n-3 PUFA adequate (Adq.) means. Fatty acids: 14:0, myristic; 16:0, palmitic; 18:0, stearic; 20:0, arachidic; 22:0, docosanoic; 16:1n-9, palmitoleic; 18:1n-(7+9), vaccenic/oleic; 20:1n-9, eicosenoic; 22:1n-9, erucic; 24:1n-9, nervonic; 1 8:2n-6, linoleic 18:3n-3, α-linolenic; 18:3n-6, γ-linolenic; 20:3n-3, eicosatrienoic; 20:4n-6, arachidonic; 22:4n-6, docosatetraenoic; 22:5n-6, docosapentaenoic; 22:5n-3, docosapentaenoic 22:6n-3, docosahexaenoic.

^aNo significant difference between respective n-3 PUFA adequate and deprived lipid pools.