Fig. 2.

Formation of FAMEs from one drop of whole blood on filter paper and purification of FAMEs formed. Lane 1, a standard mixture of SE, FAME, TG, FFA, phosphatidylethanolamine, and PC; lane 2, blood lipids; lane 3, chloroform extract after reaction; lane 4, hexane extract after reaction; lane 5, eluate of 1.5% methyl acetate/hexane from a cartridge column of silica gel that adsorbed the hexane extract. The TLC plate was first developed with chloroform/methanol/water/acetic acid (65:35:4:1, v/v/v/v) to 3.5 cm from the origin, dried in vacuo, and then redeveloped with hexane/tert-butyl methyl ether/acetic acid (90:10:0.5, v/v/v) to the top of the plate. St, sterol.