TABLE 2.
Comparison of fish oil fatty acid compositions determined by a conventional Sap/BF3 method, by a conventional anhydrous methanolic HCl method, and by the conc. HCl/methanol/toluene method
| Conventional Method |
Present Method (1.2% HCl) |
|||
|---|---|---|---|---|
| Fatty Acida | Sap/BF3 | Anhyd. HCl | 45°C/16 h | 100°C/1 h |
| % | % | |||
| 14:0 | 7.9 ± 0.0 | 7.8 ± 0.2 | 7.6 ± 0.1 | 7.5 ± 0.1 |
| 16:0 | 12.5 ± 0.0 | 12.2 ± 0.2 | 11.8 ± 0.1 | 12.0 ± 0.0 |
| 16:1 | 6.6 ± 0.0 | 6.6 ± 0.1 | 6.3 ± 0.1 | 6.5 ± 0.0 |
| 18:0 | 2.0 ± 0.0 | 1.8 ± 0.0 | 1.8 ± 0.0 | 1.8 ± 0.0 |
| 18:1 | 6.0 ± 0.0 | 5.8 ± 0.1 | 5.8 ± 0.1 | 5.8 ± 0.0 |
| 18:2 n-6 | 1.5 ± 0.0 | 1.5 ± 0.0 | 1.5 ± 0.0 | 1.5 ± 0.0 |
| 18:3 n-3 | 1.4 ± 0.1 | 1.3 ± 0.0 | 1.3 ± 0.0 | 1.3 ± 0.0 |
| 18:4 n-3 | 4.5 ± 0.0 | 4.8 ± 0.1 | 4.7 ± 0.1 | 4.8 ± 0.0 |
| 20:1 | 15.1 ± 0.1 | 14.6 ± 0.0 | 14.7 ± 0.1 | 14.7 ± 0.1 |
| 20:4 n-6 | 0.6 ± 0.0 | 0.7 ± 0.0 | 0.6 ± 0.0 | 0.6 ± 0.0 |
| 20:4 n-3 | 1.1 ± 0.1 | 1.1 ± 0.1 | 1.0 ± 0.0 | 1.1 ± 0.0 |
| 20:5 n-3 | 9.2 ± 0.1 | 10.1 ± 0.0 | 10.0 ± 0.0 | 10.0 ± 0.1 |
| 22:1 | 20.3 ± 0.3 | 19.5 ± 0.4 | 20.4 ± 0.5 | 19.8 ± 0.1 |
| 21:5 n-3 | 0.4 ± 0.0 | 0.4 ± 0.0 | 0.4 ± 0.0 | 0.4 ± 0.1 |
| 22:5 n-3 | 1.7 ± 0.1 | 1.7 ± 0.1 | 1.7 ± 0.1 | 1.7 ± 0.0 |
| 22:6 n-3 | 9.4 ± 0.1 | 10.4 ± 0.2 | 10.4 ± 0.2 | 10.5 ± 0.2 |
Each value is the average of three determinations.
a
Fatty acids are designated by number of carbon atoms:number of double bonds.