TABLE 1.
Standard curves and recovery percentages of MeOH method for extraction of additions of standard LPLs to human serum or plasma samples
| Analyte | Concentration Added, µM | ConcentrationMeasured (n = 3), µMc | Recovery of Added Analyte, %c | Linear Regression Equation |
|---|---|---|---|---|
| S1Pa | 0 | 0.83 (0.03) | y = 0.5237x + 0.0087 (r2 = 0.9978) | |
| 0.2 | 1.03 (0.04) | 98.6 (1.3) | ||
| 0.5 | 1.33 (0.11) | 100.6 (1.3) | ||
| 1 | 1.83 (0.04) | 100.2 (2.3) | ||
| 2 | 2.95 (0.16) | 106.0 (2.8) | ||
| 16:0 LPAa | 0 | 0.72 (0.08) | y = 1.5188x - 0.0053 (r2 = 0.9989) | |
| 0.5 | 1.21 (0.20) | 98.3 (3.4) | ||
| 1 | 1.74 (0.12) | 102.4 (2.6) | ||
| 2 | 2.66 (0.10) | 97.2 (2.8) | ||
| 3 | 3.74 (0.23) | 100.6 (0.9) | ||
| 18:1 LPAa | 0 | 0.81 (0.06) | y = 1.300x - 0.021 (r2 = 0.9902) | |
| 0.5 | 1.24 (0.06) | 86.2 (4.5) | ||
| 1 | 1.65 (0.10) | 84.1 (4.1) | ||
| 2 | 2.85 (0.11) | 102.0 (4.9) | ||
| 3 | 4.06 (0.20) | 108.5 (4.8) | ||
| 18: 0 LPAa | 0 | 0.20 (0.04) | y = 0.7924x + 0.0232 (r2 = 0.9938) | |
| 0.2 | 0.37 (0.08) | 86.7 (2.3) | ||
| 0.5 | 0.68 (0.03) | 95.0 (2.5) | ||
| 1 | 1.20 (0.10) | 99.4 (3.6) | ||
| 2 | 2.39 (0.12) | 109.3 (4.5) | ||
| 16:0 LPCb | 0 | 67.7 (1.3) | y = 0.6796x + 0.0123 (r2 = 0.9981) | |
| 10 | 78.0 (2.4) | 103.4 (2.5) | ||
| 25 | 94.9 (3.1) | 108.8 (3.3) | ||
| 50 | 118.1 (2.5) | 100.8 (1.2) | ||
| 100 | 153.8 (4.3) | 86.2 (1.2) | ||
| 18:1 LPCb | 0 | 14.3 (0.6) | y = 0.6011x + 0.0104 (r2 = 0.9966) | |
| 5 | 20.1 (1.1) | 114.8 (4.6) | ||
| 10 | 24.5 (0.5) | 101.8 (1.3) | ||
| 25 | 41.0 (0.2) | 106.5 (2.3) | ||
| 50 | 68.5 (1.4) | 108.2 (1.2) | ||
| 18:0 LPCb | 0 | 22.9 (0.8) | y = 0.8153x + 0.0058 (r2 = 0.9974) | |
| 5 | 27.9 (1.3) | 100.3 (0.3) | ||
| 10 | 32.9 (1.5) | 99.8 (1.2) | ||
| 25 | 51.6 (2.1) | 114.8 (3.3) | ||
| 50 | 72.4 (1.6) | 99.0 (2.5) |
a
Extracted lipids from 10 µl of serum samples, and the internal standard was 14:0 LPA (10 pmol);
b
Extracted lipids from 2 µl of plasma samples, and the internal standard was 12:0 LPC (100 pmol);
c
Data are presented as the mean (SD).