TABLE 2.
Comparisons of the recoveries of different methods for extraction of S1P and LPAs
| MRM Transition Pair |
BD Methodc |
Modified BD Methodc |
Butanol Method 1c |
Butanol Method 2c |
||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Compound | Parent Ion | Daughter Ion | Meana | SDb | Meana | SDb | Meana | SDb | Meana | SDb |
| S1P | 378.0 | 79 | 3.2 | 0.7 | 37.0 | 0.2 | 64.8 | 2.4 | 97.1 | 15.3 |
| LPAs | ||||||||||
| 16:0 | 409.0 | 153 | 6.6 | 1.9 | 128.5 | 9.2 | 165.6 | 11.2 | 113.1 | 0.2 |
| 18:2 | 433.0 | 153 | 8.6 | 2.1 | 134.3 | 1.3 | 185.1 | 10.1 | 89.1 | 2.0 |
| 18:1 | 435.0 | 153 | 11.3 | 2.7 | 119.6 | 3.6 | 166.4 | 20.9 | 111.0 | 2.4 |
| 18:0 | 437.0 | 153 | 25.1 | 0.6 | 138.5 | 4.5 | 248.5 | 116.0 | 109.2 | 5.8 |
| 20:4 | 457.0 | 153 | 14.6 | 4.3 | 161.4 | 6.3 | 208.1 | 12.3 | 78.7 | 0.9 |
| 22:6 | 481.0 | 153 | 18.9 | 6.9 | 168.5 | 26.5 | 199.5 | 27.7 | 47.6 | 5.8 |
a
Peak area obtained by MS analysis of lipids extracted by Butanol method, BD method and modified BD method, respectively, as percent of that obtained by MS analysis of lipids extracted by MeOH method.
b
SD.
c
10 µl of serum were used for extraction of lipids.