TABLE 1.
Properties of the synthetic standards mixture, sn1/sn2 ratios, and extraction efficiencies
| AA Standard | Concentration* (nM) | sn1/sn2 Ratio | Extraction Efficiency (%)** | DHA Standard | Concentration* (nM) | sn1/sn2 Ratio | Extraction Efficiency (%)** |
|---|---|---|---|---|---|---|---|
| 17:0/20:4-PC | 221 | 0.37 | 100.0 | 21:0/22:6-PC | 173 | 0.30 | 99.8 |
| 17:0/20:4-PE | 10 | 0.38 | 88.1 | 21:0/22:6-PE | 49 | 0.38 | 97.5 |
| 17:0/20:4-PG | 12 | 0.33 | 99.6 | 21:0/22:6-PG | 10 | 0.35 | 99.9 |
| 17:0/20:4-PI | 19 | 0.56 | 100.0 | 21:0/22:6-PI | 16 | 0.57 | 99.9 |
| 17:0/20:4-PS | 107 | 2.24 | 94.5 | 21:0/22:6-PS | 438 | 2.21 | 99.3 |
| 17:0/20:4-PA | 113 | 3.15 | 100.0 | 21:0/22:6-PA | 117 | 3.06 | 98.9 |
*
The nominal concentration of each species, based on the manufacturer's label.
**
Calculated as O/(A+O), where A and O are the transition signal recorded in the aqueous and organic phases, respectively.