Figure 3.
HAd5-E1A and AdCMV-pureCCK effects on mouse, human, and rat islet cell proliferation. [3H]Thymidine incorporation assays in mouse (A), human (B), and rat (C) islets. Islets were untreated or treated with control adenovirus (β-gal or green fluorescent protein), AdCMV-pureCCK (CCK), or HAd5-E1A (E1A). In mouse islets (A), only HAd5-E1A stimulated islet cell proliferation (7.3-fold; P < 0.01). In human islets (B), again only HAd5-E1A stimulated islet cell replication (19.6-fold, P < 0.01). In rat islets (C), HAd5 and AdCMV-pureCCK stimulated islet cell proliferation by 18.4-fold (P = 0.01) and 5.7-fold (P < 0.02), respectively. Comparisons were made by Student's paired t test.