Table 1.
AhR agonist TCDD markedly enhances expression of κ-casein in breast cancer cells
| Fold induction of κ-casein in four breast cancer cell lines
|
|
|
|
|---|---|---|---|
| Veh | TCDDa | 9-CisRA | |
| MDA MB-231 | 1 | 137 | 346b |
| SKBR3 | 1 | 5.64 × 108 | 1.76 × 109 |
| MCF-7 | 1b | 3.43 × 107 | 1.01 × 108 |
| ZR-75-1 | 1 | 1.75 × 107 | 4.57 × 107 |
qPCR analysis of endogenous κ-casein expression. MDA MB-231 cells were treated for 48 h with Veh, TCDD (10 nm) or SAHA (500 nm). SKBR3, MCF-7, and ZR-75-1 cells were treated for 48 h with Veh, TCDD (10 nm), or 1 mm 9-cisRA. Cells were harvested for total RNA, which was used for qPCR. Tabular data are represented as fold induction over vehicle for each cell line (set at 1). Data represent the average of triplicate amplification reactions for each condition in a representative experiment (n = 3 independent assays).
a
P < 0.01 for comparison between Veh and TCDD.
b
For MDA MB-231 cells 500 nm SAHA was substituted for 9-CisRA.