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. 2010 Jan 15;24(2):447–463. doi: 10.1210/me.2009-0295

Figure 4.

Figure 4

Effect of mir-100 on mTOR signaling and RAD001 sensitivity in clear cell ovarian cancer cells. A, Protein levels of mTOR and phosphorylated isoforms of 4EBP1 and p70 S6 kinase were examined by Western blot on OVSAYO cell lysates after transduction of a lentiviral vector expressing mir-100 or a nontargeting sequence (negative control). β-actin levels served as a loading control. B, Viability of OVSAYO cells treated with varying concentrations of RAD001 after stable transduction with mir-100 or a negative control vector. Student's t test: *, P < 0.001; **, P < 0.0001; ***, P < 0.00001.