Table 2.
All mir-100 predicted target genes that were overexpressed in clear cell ovarian cancer
| Gene | Description | Microarray fold change (clear cell vs. NOSE) | Microarray fold change (mir-100 overexpression) | Algorithm |
|---|---|---|---|---|
| FGFR3 | Fibroblast growth factor receptor 3 | +13.10 | −2.62 | T, P, M |
| FRAP1/mTORa | FK506-binding protein 12-rapamycin associated protein 1 | +1.92 | −1.85 | T, P, M |
| CTDSPL | CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) small phosphatase-like | +3.86 | −1.62 | T, P, M |
| TRIB1 | Tribbles homolog 1 (Drosophila) | +3.47 | −1.28 | T |
| CMTM6 | CKLF-like MARVEL transmembrane domain containing 6 | +3.12 | −1.15 | M |
| HLTF | Helicase-like transcription factor | +2.09 | −1.12 | M |
| EIF2C2/AGO2 | Eukaryotic translation initiation factor 2C, 2 | +3.29 | −1.07 | T, P, M |
| IMPDH1 | IMP (inosine monophosphate) dehydrogenase 1 | +2.31 | −1.07 | M |
| RAVER2 | Ribonucleoprotein, PTB-binding 2 | +2.44 | −1.06 | T, P, M |
| CCDC21 | Coiled-coil domain containing 21 | +2.30 | +1.04 | T |
| KAZALD1 | Kazal-type serine peptidase inhibitor domain 1 | +4.32 | +1.06 | M |
| E2F2 | E2F transcription factor 2 | +6.76 | +1.07 | M |
| CDC25A | Cell division cycle 25 homolog A (S. pombe) | +3.06 | +1.61 | M |
| PLAGL2 | Pleiomorphic adenoma gene-like 2 | +3.82 | +1.63 | M |
Nine of 14 genes were down-regulated after mir-100 overexpression. Prediction algorithms: T, TargetScan; P, PicTar; M, miRanda.
a
Although FRAP1/mTOR was less than 2-fold up-regulated in clear cell vs. NOSE, it was included in our analysis based on previous work validating the gene as a bona fide mir-100 target (44).