Figure 2.
ERαLysM −/− mice express lower levels of ERα in macrophages and osteoclasts. A–D, Quantitative RT-PCR analysis of mRNA from: A, macrophages formed from nonadherent bone marrow cells cultured for 4 d in the present of M-CSF. B, Mature osteoclasts generated from bone marrow cells cultured with M-CSF and RANKL for 5 d. C, Osteoblastic cells derived from calvaria of 2- to 3-month-old mice. D, Soft tissues harvested from 12-wk-old mice (n = 5). Bars, Mean ± sd, *, P < 0.05. In panels A and B, the mean was calculated using cells from three individual animals and triplicate determinations per animal. In panel C, the mean was calculated from triplicate determinations obtained from cells pooled from three animals.