Figure 2.

TRH mRNA is expressed in the DMH. ISH using S³⁵ radiolabeled probes. A, ISH was performed on brain slices from euthyroid, hypothyroid, and hyperthyroid WT mice using the TRH mRNA (left column) and TRH hnRNA (right column) probes. Magnification, ×10; scale bars per column, 100 μm. n = 4 mice for each probe under each condition. B, Quantification of ISH via pixel density. Data are presented as sample means relative to control euthyroid mice ± sem. Significance was tested within each probe via one-way ANOVA.