A–D, Representative cross-sectional μCT generated images of the midshaft cortical bone from femurs of WT (A), Klotho (B), HYP (C), and Klotho/HYP (D) mice; E–H, bar graphs show the derived values for the μCT analysis for cortical mask (cross-sectional area inclusive of porosity) (E), cortical bone area (exclusive of porosity) (F), cortical thickness (G), and cortical porosity (H). Different letters indicate P < 0.05 between the two groups. n = 6 for all groups. Differing lowercase letters indicate statistically significant differences (P < 0.05) between the groups. Bars with the same letters are not significantly different.