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. 2009 Dec 18;151(2):671–682. doi: 10.1210/en.2009-0711

Figure 4.

Figure 4

Nuclear GR immunoreactivity is enhanced in the hypothalamus of JNK1-deficient mice. A and B, Immunofluorescence analysis of GR-positive cells (A) in fed and 36-h-fasted controls and JNK1 mutants and its quantification (B). Hypothalamic sections were selected from Bregma −2.06 to −2.30. n = 10–16 sections from three mice per group. C, mRNA expression of GR gene (NR3C1) was measured by semiquantitative RT-PCR from fed (n = 5–6) and 36-h-fasted (n = 4–8) mice. β-Actin was used as an internal control. Expression was normalized to control fed value. D, Plasma corticosterone (cort) levels from fed and 36-h-fasted mice were measured by enzyme immunoassay. Control n = 8; mutant n = 6. E, mRNA expression of hypothalamic Crh was measured by semiquantitative RT-PCR from fed and 36-h-fasted mice. n = 5–6. β-Actin was used as an internal control. Expression was normalized to control fed value. *, P < 0.05; **, P < 0.01 as determined by Student’s t test.