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Pyrosequencing analysis of genomic DNA from female and male mouse cortex collected at PND 4, 18, and 25 (n = 6–8 per time point). A and B, Percent methylation of CG sites found in exon A in female (A) and male (B) cortex; C and D, percent methylation of CG sites found in exon C of the ERα gene in female (C) and male (D) cortex. Asterisks indicate significant differences from PND 4 determined by Tukey post hoc tests after a two-way ANOVA (P < 0.05).
