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. 2009 Dec 16;151(2):606–616. doi: 10.1210/en.2009-1135

Figure 3.

Figure 3

LIF activates POMC-EGFP neurons in the hypothalamus. A, D, and G, POMC-EGFP expression in the arcuate nucleus. B, Low cFos IR (red) in vehicle-treated mice (n = 3). C, Few POMC neurons show nuclear cFos IR after vehicle treatment. E and H, cFos IR is increased in LIF-treated (10 ng, n = 4) mice. F and I, LIF increases cFos IR in POMC neurons. G–I, Enlargement of D–F shown for clarity (white boxes indicate regions of enlargement). J, LIF increases cFos IR per section. K, Approximately 30% of POMC neurons contain cFos immunoreactivity after either 10 or 100 ng LIF treatment. Data are expressed as mean ± sem, and statistics calculated by one-way ANOVA followed by a post hoc analysis using a Bonferroni corrected t test (***, P < 0.001 vs. vehicle). Scale bars, 100 mm (A–F) and 50 mm (G–I). 3V, Third ventricle.