Figure 5.

Panel A, Western blot analysis shows decreased phospho-Akt and G6PDH levels in aged gastrocnemius muscles compared with that of young muscles. Supplementation with 1 cm testosterone (T) prevents such age-related decrease in phospho-Akt and G6PDH levels. The gels are representative of two animals at each group from one of three separate experiments. GAPDH in the immunoblot is shown as a loading control. Panels B and C, Quantification of band intensities shows complete restoration of phospho-Akt and G6PDH, respectively, to their young levels after treatment with 1 cm testosterone. Values are mean ± sem. Means with unlike letters are significantly (P < 0.05) different.