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. 2010 Jan 5;151(2):617–627. doi: 10.1210/en.2009-0978

Figure 3.

Figure 3

RFRP peptide and its receptor proteins are expressed at specific stages during the seminiferous cycle. Triple-label immunohistochemistry staining for RFRP/GPR147/DAPI (A) and RFRP/GPR74/DAPI (B) in hamster testis reveals a temporal expression pattern during the seminiferous cycle. Spermatids at various steps of spermiogenesis are assigned numbers 1–17 as described in the text. RFRP ligand (A and B, green) is expressed in tubules from stages VIII to XIII surrounding the nucleus of both spermatocytes and spermatids during steps 8–13. GPR147 receptors (A, red) were found in myoid cells (Mi) of all tubules, P primary spermatocytes from stages VIII to XII, the MD spermatocytes in stage XIII, round spermatids a during steps 6–7 and elongated spermatids at during steps 16–17 from stages VI to VII. GPR74 receptors (B, red) were only found in elongated spermatids at late steps 16–17 from stages VI to VII. Cell nuclei were stained by DAPI (blue or white). The DAPI channel is shown in the right panel. An asterisk indicates the lumen side of the tubule.