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. 2010 Jan 5;151(2):617–627. doi: 10.1210/en.2009-0978

Figure 5.

Figure 5

RFRP peptide and its receptor proteins are suppressed in SD-R animals. Triple-label immunohistochemistry staining for RFRP/GPR147/DAPI (A) and RFRP/GPR74/DAPI (B) in the testes of LD, SD-R, and SD-NR hamsters. For all genes, SD-NR animals showed a similar expression pattern to LD animals. Minimal RFRP expression (A and B, green) was seen in SD-R animals. GPR147 receptor (A, red) can be found in myoid cells (Mi) of all tubules in LD, SD-R, and SD-NR animals. In SD-R animals, P, primary spermatocytes, and MD spermatocytes exhibited GPR147 expression. GPR174 expression was not seen in round spermatids (Sd) in SD-R animals. GPR74 expression was found in not observed in SD-R animals. Spermatids at different steps of spermiogenesis were assigned values of 1–17. Cell nuclei were stained by DAPI (blue or white). The DAPI channel (white) is shown in the right panel. An asterisk indicates the lumen side of the tubule.