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IEF in ampholyte gradient of pH 3 to 10 with crude extracts. Lane 1, enzymes (pIs, 5.4 & ≈ 9.0) from E. aerogenes (EaN146); lane 2, enzyme (pI 8.2) from the clone (CN146) and lane 3, enzymes (pIs, 5.4 & ≈ 8.9) from the transformant (TN146). The enzymes at pI 9 in lane 1 and pI 8.9 in lane 3 represented chromosomal enzymes (AmpC).
