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. 2010 Jan 6;9:1. doi: 10.1186/1476-511X-9-1

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Copyright ©2010 Niwa et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Cytokine release upon OVA challenge in DO11.10 RAG1^-/-mouse lines. Cerk^-/-, Sphk1^-/-and Sphk2^-/-and WT mice all in the genetic background DO11.10 RAG1^-/-, were challenged with OVA as described in the Experimental section. Blood samples were collected after 2 h and 4 h for cytokine measurement and serum was prepared. Serum cytokine levels shown are the mean ± SD of the 2 h together with the 4 h measurements expressed as a percentage of WT levels, from 3 independent experiments with a total of 24 animals per group.