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TPEF ratio of hMSCs. Fluorescence ratio of hMSCs after 21 days of culture in propagation medium remains high (panel A), while hMSCs in adipogenic medium (panel B) have a lower ratio. (C) The populations of hMSCs in propagation medium (triangles) and adipogenic medium (circles) can be differentiated by plotting the fluorescence ratio against normalized area [calculated as (1 − eccentricity) × area in thousands of pixels]. Reproduced with permission from Rice et al. 2007.65
