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Dependence of image resolution on NA. TPEF (at 800 nm) image stack of a silk scaffold acquired with a (A) 20×, 0.7 NA objective (750 × 750 × 200 μm) and (B) 63×, 1.2 NA water immersion objective (238 × 238 × 200 μm). TPEF (at 740 nm) image sections of J2 fibroblasts acquired through a (C) 20×, 0.7 NA objective and (D) 63×, 1.2 NA water immersion objective. Bar = 75 μm.
