Table 2.
GOX enzymatic activities.
| Construction(a) | Final Biomassb | GOX activity(c) | ||||||
|---|---|---|---|---|---|---|---|---|
| Supernatant | Cell wall | Cell-bound | Total | |||||
| (gDW/L) | U/mgProt | U/gDW | U/mgProt | U/gDW | U/mg Prot | U/gDW | U/gDW | |
| Km1 | 7.4 ± 0.3 | 0.3 ± 0.1 | 0.5 ± 0.1 | 2.4 ± 0.1 | 1.9 ± 0.1 | 0.03 ± 0.02 | 4.0 ± 1.4 | 7.4 ± 2.5 |
| Km2 | 12.1 ± 0.2 | 251 ± 72 | 407 ± 16 | 180 ± 24 | 1145 ± 35 | 1.3 ± 0.2 | 171 ± 44 | 1722 ± 62 |
| Km3 | 12.0 ± 0.1 | 171 ± 44 | 255 ± 1 | 187 ± 8 | 850 ± 70 | 2.4 ± 0.3 | 291 ± 27 | 1395 ± 42 |
| Kl1 | 9.9 ± 0.2 | 40 ± 3 | 161 ± 17 | 16 ± 1 | 13 ± 1 | 0.3 ± 0.1 | 31 ± 2 | 205 ± 16 |
| Kl2 | 11.2 ± 0.0 | 32 ± 1 | 94 ± 6 | 64 ± 5 | 49 ± 8 | 0.1 ± 0.0 | 16 ± 3 | 158 ± 17 |
| Kl3 | 9.6 ± 0.2 | 29 ± 1 | 94 ± 12 | 76 ± 4 | 64 ± 0 | 0.1 ± 0.0 | 13 ± 1 | 170 ± 11 |
Heterologous GOX production in the supernatant, cell wall and cell-bound fractions of different K. marxianus and K. lactis strains.
(a) see Materials and Methods section, Table 1 and Figure 1 for a description of the genetic constructions.
(b) see Materials and Methods section, for a description of the cultivation conditions.
(c) subcellular distribution according to Rouwenhorst et al. [50]