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. 2010 Jan 21;9:4. doi: 10.1186/1475-2859-9-4

Table 3.

GOX distribution.

Construction(a) GOX activity per g DW in each fraction relative to the total(b, c)

Supernatant Cell wall Cell-bound
Km1 9% ± 3% 31% ± 8% 62% ± 11%
Km2 23% ± 2% 67% ± 1% 10% ± 3%
Km3 19% ± 1% 61% ± 3% 21% ± 2%
Kl1 79% ± 2% 6% ± 0% 16% ± 2%
Kl2 60% ± 2% 31% ± 2% 10% ± 0%
Kl3 50% ± 3% 38% ± 3% 10% ± 3%

Subcellular distribution of heterologous GOX in different K. marxianus and K. lactis strains.

(a) see Materials and Methods section, Table 1 and Figure 1 for a description of the genetic constructions.

(b) see Materials and Methods section, for a description of the cultivation conditions.

(c) subcellular distribution according to Rouwenhorst et al. [50]