Table 3.
Construction(a) | GOX activity per g DW in each fraction relative to the total(b, c) | ||
---|---|---|---|
Supernatant | Cell wall | Cell-bound | |
Km1 | 9% ± 3% | 31% ± 8% | 62% ± 11% |
Km2 | 23% ± 2% | 67% ± 1% | 10% ± 3% |
Km3 | 19% ± 1% | 61% ± 3% | 21% ± 2% |
Kl1 | 79% ± 2% | 6% ± 0% | 16% ± 2% |
Kl2 | 60% ± 2% | 31% ± 2% | 10% ± 0% |
Kl3 | 50% ± 3% | 38% ± 3% | 10% ± 3% |
Subcellular distribution of heterologous GOX in different K. marxianus and K. lactis strains.
(a) see Materials and Methods section, Table 1 and Figure 1 for a description of the genetic constructions.
(b) see Materials and Methods section, for a description of the cultivation conditions.
(c) subcellular distribution according to Rouwenhorst et al. [50]